Extracellular Products of Endophytic Fungi Isolate C.1.1 And C.3.3 | Shirly Kumala

Summary :
⁣Michelia champaca L. is known to have efficacy as an antimicrobial, antioxidant, and anticancer. In general, endophytic fungi that live in plant tissues is capable of producing secondary metabolites as their host plant. The objective of this research is to examine the antimicrobial potential of endophytic fungi isolates C.1.1 and C.3.3 from (Michelia champaca L.). Method first isolates obtained from previous research was rejuvenated on PDA (Potato Dextrose Agar). Fermentation was carried out on each isolate for 12 days. The fermented supernatant was then extracted partially with n-hexane, ethyl acetate, and n-butanol solvent. Finally the extract was then concentrated and tested for antimicrobial activity using microdilution methods. The antimicrobial activity result showed that n butanol extract of Champaca branches was able to inhibit the microbial growth with Minimum Inhibitory Concentration(MIC) and Minimum Bactericidal Concentration (MBC) in Staphylococcus aureus ATCC 6538 (12.5%), Escherichia coli ATCC 8739 (12.5%), Methycillin Resistant Staphylococcus aureus (12.5%) and for the Candida albicans ATCC10231 (50%) in isolate C1.1. However isolate C.3.3 had less activity in Staphylococcus aureus ATCC 6538 with MIC and MBC 25%. The conclusion, both endophytic fungi isolates of Michelia champaca branches had the potential to inhibit the growth of pathogenic microbes.


About Author :
⁣Dr. Shirly Kumala has completed her PhD from Biomedical Faculty, University of Indonesia, Jakarta. She is the Dean of Pharmacy Faculty, Universitas Pancasila, Jakarta, Indonesia. She has published more than 28 papers both International and National Journals and has been serving as a reviewer in Journal of Pharmacy.